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Practical HPLC Method Development  
 
 
Course Duration:
3 days, 9am - 5pm
 
Course Fee:
£695.00
 
What's Included:
Lunch, tea/coffee, notes, course certificate
 
Suitable For:
HPLC Users with at least 6 months hands-on experience
 
Location:
Laserchrom, Rochester, Kent. UK
 
Course Presenter:
 
Course Accreditation:
 
Institute of Biomedical Scientists
 
Royal College of Pathologists
 
Institute of Science & Technology
 
Next course date:
See this colour in the Course Calendar
 

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A systematic and comprehensive approach to HPLC Method Development. The practical and teaching sessions run concurrently on this course, and over the course of the three days we develop a method from nothing. We show how to decide if the method should be isocratic or a gradient, which column to use, which detection method to use, and which stating conditions to try. Then over the three days we optimise the organic solvent, showing how to exploit the selectivity options of the available solvents. We optimise for temperature, demonstrate the need for ion suppression, and extensively show the effect of using a pH buffer at a range of pH. We cover the selection of the buffer, and the choice of buffer concentration. Gradient elution is discussed in detail.

At the end of the course we discuss a series of examples of methods which have been developed in the laboratories at Laserchrom, and explain how some specific and challenging problems were overcome. The course ends with an written exercise and a discussion of the answers.

 

 
                     
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