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Principles of HPLC Series


1: Introduction to HPLC


The Principles of HPLC Series is a set of one-day courses which can be run anywhere in the world at an affordable price. In the UK they are run in Bristol, Warrington and Rochester.

These courses do not include practical elements, so for those with no experience at all of HPLC, we recommend the Practical HPLC Series. However if you use HPLC but need to understand the operating principles, this series of courses is for you.


The Introduction to HPLC Course covers reversed phase columns (eg C18/ODS), the basic theory of HPLC, an overview of HPLC equipment, how to set up and follow an isocratic method, basic integration, and exterenal standard calibration.

Following the course a participant should be able to recognise the components of an HPLC system, run an isocratic method and recognise the most commone errors.

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2: Further HPLC

3: HPLC Method Development

Following directly from the Introduction to HPLC Course, Further HPLC covers further principles of HPLC, a wider range of columns, an introduction to gradient elution and calibration using an internal standard.

Following the course, participants should be able to set up and run a gradient method, and understand the principles of internal standard calibration.

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This course covers the basis for using a systematic approach to HPLC Method Development. We consider what needs to be optimised and in what order, to achieve a robust and successful HPLC method.

Included in this is optimisation of the column, the eluent composition, temperature, pH, buffer choice and buffer concentration. We thedn look at ways to run the method faster without losing resolution.

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4: HPLC Troubleshooting

5: Advanced HPLC


This course covers troubleshooting an HPLC system from the symptoms observed in the chromatogram and from the instrument, such as baseline noise, back pressure, retention times, peak height and shape, the void volume peak etc.

By the end of the course participants should be able to troubleshoot most commonly observed faults correctly.

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Unlike the other four course, Advanced HPLC is a full week long and is suitable only for those with at least a year or two's hPLC experience. It contains something for even the most experienced chromatographer, and it is only available as an on-site course. If you book this course, we recommend you arrange a time when your whole HPLC team is free.

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