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Course duration: |
1 day, 9am - 5pm |
| Cost: |
£195.00 + VAT |
| Suitable for: |
Anyone with little or no HPLC experience, no chemistry knowledge required |
| Location: |
Manchester, London, Bristol and Cambridge (see yellow link tab) |
| Fee includes: |
All training notes and folder, lunch, tea and coffee, course certificate |
| Course Presenter: |
Dr Stuart Jones |
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Course content:
- History of HPLC
- Basic theory of HPLC
- Brief introduction to manual injection valves, isocratic pumps and UV detection
- Make injection and run basic HPLC chromatogram
- Effects of changing eluent composition, flow rate, column and detector
- Making up an accurate mobile phase composition and degassing
- Make flow path connections
- Basic precautions to avoid problems
- HPLC solvent selection
- Column efficiency and capacity
- Normal phase versus reversed phase analysis
- Introduction to chromatographic interpretation
- Introduction to sample preparation
- Introduction to autosamplers
- Safety: Working with small particle size materials, high pressures, UV radiation and electronic safety.
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