Course Content:
Principles of GPC
Separation Principles
Column exclusion limit
Void volume and Pore volume of the system
Separation of a wide molecular weight range
Using 3 or 4 columns of different types in series
Using 3 or 4 mixed bed columns
Using 3 or 4 columns of mixed pore-size gel
Column formats in GPC
Standard columns 8.0 x 300mm
Rapid Analysis Format 6.0 x 150mm
High Resolution Format 4.6 x 250mm
Solvent Peak Separation Columns
Guard Columns
Eluent Choice
Sample solubility
Prevention of Chmical Interaction with the column
Refractive index
Sample preparation
Sample Concentration and dilution
Swelling of large molecules in solution
Molecular weight calibration
Calibration range
Calibration standards
Solvent dependence
Detection for GPC
UV
Refractive index
Evaporative Light Scattering Detectors
GPC Data Systems
Day 2 - Lab Practical |