Intermediate Practical HPLC Course - 2 days
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Course duration: |
2 days, 9am - 5pm |
| Cost: | £395.00 + VAT |
| Suitable for: | Anyone with little or no HPLC experience, but we recommend doing the Introductory course first, because material covered there is not repeated during this course. |
| Location: | Laserchrom in Rochester, Kent. |
| Fee includes: | All training notes and folder, lunch, tea and coffee, course certificate |
| Course Presenter: | Dr Stuart Jones |
Course content: HPLC Columns - Differences between different C18 packings Comparison between C8 and C18 When to use 5u, when to use 10u and when to use 3u Type A and Type B silicas Base deactivated silicas Normal phase columns - Si, CN, DIOL, NH2 Ion Exchange columns - SAX, SCX, WAX, WCX Effects of changing column length, and how to decide the best length to use. Guard columns and pre-columns Cleaning a column Understanding isocratic elution - effect of changing flow rate, effect of changing %B Gradient elution - %B range run time gradient profile effects of changing colunm length gradient dwell volume Starting a gradient with the run start. Detection - UV Diode Array Fluorescence Data handling |
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| Day 2 is a Lab Practical.
There are three practical experiments run on this course: The analysis of mono- and di-valent cations by ion chromatography Analysis using an internal standard Running a gradient method. Assessment Presentation of Course Certificates |
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| Customer Comments | ||
Course Dates in 2010 |
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| 21-22nd October | ||
| Course Accreditation for CPD Points | ||
| Royal College of Pathologists | ||
| Institute of Biomedical Scientists | ||
| Travel and Accommodation | ||
| Directions to Laserchrom | ||
| Accommodation information | ||
| Booking Information | ||
| Course Calendar | ||
| Booking Form | ||
| Terms and Condition of Booking | ||
| Free Lunchtime Seminars | ||
| Special Offers | ||