Course content:
Introduction to the chemistry of sugars
- Mono-, di-, oligo- and poly-saccharides
- triose, tetraose, pentose and hexose sugars
- How sugars combine
- Isomers and anomers
- Sugar alcohols and sugar amines
Solubility of sugars
HPLC methods of analysis
- Normal phase - amino columns
- Ligand Exchange
- Size exclusion
- Ion chromatography
Comparison of the separations with each type of column
Analysis of monosaccharides
Analysis of disaccharide
Analysis of oligosaccharaide
Analysis of polysacchraides
Detection methods
- Refractive Index
- ELSD
- Electrochemical
Analysis of sugar syrups
- Sample handling
- Solubility issues with normal phase separations
- Quantitative analysis
- Recommended columns for analysing the disaccharade element
Day 2 is a practical day in the lab, running sugar samples, inlcuding syrups and oligo mixes. |