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HPLC of Sugars Course - 2 days

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Course duration:

2 days, 9am - 5pm
Cost: £395.00 + VAT
Suitable for: Anyone with a little HPLC experience
Location:

Laserchrom, Rochester

Fee includes: Excellent training notes and folder, lunch, tea and coffee, course certificate
Course Presenter: Dr Stuart Jones
 

Summary:

Sugar analysis is complex, bcause many sugars are very similar, have almost identical polarity and no UV chromaphore. This course covers the main ways to analyse sugars, and by the end of the course the analyst should know enough to select the most appropriate approach to their sugar separation.

Course content:

Introduction to the chemistry of sugars

  • Mono-, di-, oligo- and poly-saccharides
  • triose, tetraose, pentose and hexose sugars
  • How sugars combine
  • Isomers and anomers
  • Sugar alcohols and sugar amines

Solubility of sugars

HPLC methods of analysis

  • Normal phase - amino columns
  • Ligand Exchange
  • Size exclusion
  • Ion chromatography

Comparison of the separations with each type of column

Analysis of monosaccharides

Analysis of disaccharide

Analysis of oligosaccharaide

Analysis of polysacchraides

Detection methods

  • Refractive Index
  • ELSD
  • Electrochemical

Analysis of sugar syrups

  • Sample handling
  • Solubility issues with normal phase separations
  • Quantitative analysis
  • Recommended columns for analysing the disaccharade element

Day 2 is a practical day in the lab, running sugar samples, inlcuding syrups and oligo mixes.

 
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