Course duration:

Location:

(see yellow tab for course dates)

Laserchrom - Rochester

Manchester

Birmingham

Cambridge

London

Bristol

Course content:

Overview of HPLC Equipment

Correct installation of an HPLC system, including electrical and fluid connections

Conditions required for dynamic equilibrium

Routine maintenance - replacement of pistons, piston seals, check valves, and detector lamps

Problem symptoms:

• Increasing retention time,
• Decreasing retention time,
• Unreproducible retention times.
• Integration problems
• Broad peaks,
• Tailing peaks,
• Split peaks,
• Flat top peaks,
• Ghost peaks
• No peaks
• Negative peaks
• Drifitng baseline,
• Spiking baseline,
• Noisy baseline
• Analysis of baseline noise
• High back pressure,
• Low back pressure,
• Fluctuating back pressure

Identifying 95% of faults through just six key indicators

Problem scenarios

Course Dates in 2010