AdvancedHPLC

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This is one of our most popular courses. Every chromatographer uses integration each time they use the HPLC, yet many would automatically assume that the calibration shown above is spot on! How to recognise invalid data is so important, and this course takes you through it, using examples all the way.

What actually happens when you change the peak width setting? How could this invalidate previous results? Apart from thousands of tickmarks, what else goes wrong when we use a peak threshold which is too low? When more than one peak appears in a time window, how does a data system decide which peak to select? What happens if time windows overlap, so a peak falls into two time windows simultaneously? What hapens if we use too high a data collection frequency? When is it valid to integrate by height instead of area, and what are the risks? A reference peak can be useful if retention times change, but how might this threaten a calibration? How do we decide when to accept fused peaks and when they pose too great a threat? When should we use standard addition calibration? When should we use bracketed calibration? How can we use system suitability testing to minimise errors? All this and much more is covered in detail on this course.

We encourage participants to bring a laptop, so we can load a data system and work through examples.

After the course, people describe this course as 'empowering', and it receives the best feedback of all our courses.