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AdvancedHPLC

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5 - HPLC Instrumentation
 
 
 
Course Duration:
1 day, 9am - 5pm
 
Course Fee:

£250.00 when taken alone

(See Special Offers for a 20% saving when booking all 5 modules, and On-site Courses to find out about having the Advanced courses run on your site)

 
What's Included:
Lunch, tea/coffe, notes, course certificate.
 
Suitable For:
HPLC Users with at least 1 year's hands-on experience.
 
Location:
 
Course Presenter:
 
Course Accreditation:
Institute of Biomedical Scientists
 
Royal College of Pathologists
 
Institute of Science & Technology
 
Next course date:

See this colour in the Course Calendar

 

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Advanced HPLC 5 gives an overview of the modules in an HPLC system, including those not often used. The aim is that the analyst can make intelligent choices of instrumentation, in the full knowledge of what options are available.

Would you find this course useful? You certainly would if you were about to buy a new or used HPLC system!!!

There are four types of HPLC pump, two types of pump motor, two ways to pump a gradient, many types of autosampler etc etc. Not all of them will work together, some are best at low flow rate, some will not work together at all. When should you use column switching and when to use a gradient? What pathlength flow cell is best in a UV detector? How can you reduce the baseline noise to a minimum? When should you use a dynamic mixer and when to use a static mixer? How does the compressibility setting work? How can you maintain really constant temperature? When is it useful to have a column heater that will chill as well as heat? When setting up high pressure mixing, which pump-heads should we use? How important is degassing for low flow rate pumps? How can you test the gradient mixing on a pump? How can you test the gradient dwell volume? (You need to know these if you are trying out a system!) All this and much more is covered on this course.

This course is useful to everyone, but is invaluable if you are setting up a lab or buying HPLC equipment!

 
                     
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