AdvancedHPLC

This module gives an overview of the options available when selecting a mobile phase, and a guide to developing the optimum eluent system for each separation.

Which solvent should be used to wash a column? Which solvent should be used to store a column? Which solvent to choose as a sample solvent? What are the requirements for a solvent to be usable as a reversed phase eluent? What are the requirements for a buffer salt to be appropriate for a reversed phase eluent? How can we increase retention times of ion pairs when using an ion pair reagent? What is unique about trifluoroacetic acid which makes it ideal for HPLC, and which property makes it a nightmare?

How can we determine quickly if a separation can be run isocratically on a reversed phase column?

All this and much more is covered on this course. We also cover solvent optimisation, showing why by far the most selectivity comes from the mobile phase rather than the stationary phase.

This course is really useful if starting to develop a method or optimise an existing one. It is also useful if changing from one column type to another. For users wanting to feel totally in control of an HPLC method, this course explains why things change and how to induce a required change.