This module is part of the Masterclass in Modern HPLC programme.

This is a rolling programme of six modules, which are run once every two months at six locations around the UK. Each module is complete in isolation, and there is no need to do the modules in any particular order. Simply select your preferred location, and in the following 12 months you will complete the course.

£250 + VAT when taken alone.

Book the full Masterclass in Modern HPLC and pay just £1095.00 + VAT for all six modules!

Locations Available:

Birmingham

Bristol

Cambridge

Cardiff

Edinburgh

Manchester

London

Other locations can be arranged if required

Summary:

A guide to the use of HPLC Solvents.

• Purity
• Viscosity
• Eluent Strength for normal phase and reversed phase
• Refractive Index
• Miscibility with other solvents
• Miscibility with water
• Sample solubility
• Buffer solubility
• Specific grades available
• UV transparency and UV cut-off
• Shelf life
• Specific Hazards - Toxicity, Flammability, Evaporation Rate
• Acid/Base/Dipole Selectivity Properties
• Compatibility with HPLC Instrumentation

Solvents to use as sample solvent

Solvents to use for sample extraction

Solvents to use for column cleaning

Solvents to use for column storage

A guide to the use of pH Buffers

• The importance of pH in HPLC
• When to use Ion Suppression and when to buffer
• pKa
• How a buffer works
• Selection of buffer concentration
• Which buffer to use at which pH
• Specifics about phosphates, acetates, borates, tris, amines, formates, citrates, glycines and ammonia
• UV cut-off
• Shelf life and storage
• Making up buffers - calculating concentration and adjusting pH
• Compatibilty with HPLC Instrumentation

Recommended Eluents

• Normal phase
• Reversed phase
• Ion exchange
• Ion chromatography
• GPC
• GFC
• LCMS

Making up an accurate mobile phase

Degassing

Effect of changing eluent composition

A guide to Ion Pair Reagents

• Anionic and Cationic
• Solubility
• Effect of alkyl chain length
• Use in enhancing detection
• Effect on equilibration times
• Use with gradient elution
• Concentration to use
• Troubleshooting

Eluent Optimisation

• Systematic solvent Optimisation using only 7 injections
• Optimisation of flow rate

Safety, Risk Assessment, Storage and Disposal.

Course Dates in 2010