This module is part of the Masterclass in Modern HPLC programme.

This is a rolling programme of six modules, which are run once every two months at six locations around the UK. Each module is complete in isolation, and there is no need to do the modules in any particular order. Simply select your preferred location, and in the following 12 months you will complete the course.

£250 + VAT when taken alone.

Book the full Masterclass in Modern HPLC and pay just £1095.00  + VAT for all six modules!

Locations Available:

Birmingham

Bristol

Cambridge

Edinburgh

Manchester

London

Summary:

This module gives an overview of the detection methods available to the HPLC analyst, discussing each in detail, with reference to sensitivity, selectivity and application.

Detector functions applicable to all detectors

• Noise
• Signal:Noise Ratio
• Drift
• Sensitivity
• Linearity
• Dynamic Range
• Response Time
• Analogue and Digital Output
• Use of Autozero

UV

• Samples which absorb UV
• Wavelength
• Eluent choice to avoid UV cut-off
• Optical Layout
• Wavelength control
• Flow cells
• Lamp and cell repalcement
• Wavelength programming
• Lamp types and expected lifetime
• Dual wavelength detection
• Spectral scanning

Diode Array (PDA or DAD)

• Number of diodes
• Advantages of diode array
• Time slice spectra
• Use of Spectral library for peak identification
• Peak Purity assessment
• Contour Maps
• 3D displays

Fluorescence

• Introduction to fluorescence
• Lamp types
• Flow cell types and replacement
• Sensitivity
• Selectivity
• Determination of the excitation and emmission wavelengths
• Fluorescence derivatisation reagents
• Pre-column derivatisation
• Post Column Derivatisation
• On-column Derivatisation

Refractive Index

• Flushing the reference cell
• Sensitivity
• Almost universal detection
• Eluent choice
• Dealing with negative peaks
• Importance of temperature control
• Mixing isocratic eluents with a gradient pump
• Importance of degassing
• Differences in sensitivity between different models
• Incompatibility with gradient elution

Evaporative Light Scattering

• Operating principles
• Gas supply requirements
• Nebulisation
• Light source
• Optimising detector sensitivity
• Eluent compatibilty
• Cleaning the evaporation chamber
• Troubleshooting

Electrochemical

• Operating principles
• Analogy with UV detection
• Electrode materials
• Coulometric and Amperometric detection
• Pulsed Amperometric detection
• Selection of applied potential
• Eluent selection
• Use with gradients
• Pump requirements
• Selectivity and applications

Conductivity

• Chemical and electronic suppression
• Effect of changing temperature
• Detection limits
• Optimising the eluent
• Sensitivity
• Limitations with certain eluents

Mass Spectrometer

• Principles of Mass Spec
• Peak identification
• Characterisation of unknowns
• Interfaces and ionisation Sources
• Mass Analysers
• Fragmentation Patterns
• Isotopes
• Molecular ions
• Single Ion Monitoring
• Quantitation

Course Dates in 2010