Masterclass in Modern HPLC

Masterclass 2 - HPLC Columns

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This module is part of the Masterclass in Modern HPLC programme.

This is a rolling programme of six modules, which are run once every two months at six locations around the UK. Each module is complete in isolation, and there is no need to do the modules in any particular order. Simply select your preferred location, and in the following 12 months you will complete the course.

Duration: 1 day
Cost:

£250 + VAT when taken alone.

Book the full Masterclass in Modern HPLC and pay just £1095.00 + VAT for all six modules!

Suitable for: Anyone with at least 6 months experience with HPLC

Locations Available:

Birmingham

Bristol

Cambridge

Edinburgh

Manchester

London

Course Presenter: Dr Stuart Jones
 

Summary:

This module gives an overview of the columns available in HPLC. It is not a guide to column manufacturers although many brands of column are referenced in the course. The aim is to enable an HPLC analyst to be able to make an intelligent choice when selecting columns for a specific application.
Course Content:

Column Packing Material

  • Silica
  • Alumina
  • Polymers
  • Zirconia
  • Carbon
  • Monolithic silica
  • Particle Size

Bonded phases

  • C18/ODS
  • C8
  • C4/C6/C1
  • End Capping
  • Polar Embedded Phases
  • Amino
  • Cyano
  • DIOL
  • Base Deactivated Phases

Normal Phase and reversed Phased

Using Polymer-based Reversed Phase columns

Column Sizes and Shapes

Column Loading Capacity

Scale down to microbore & narrowbore

Chiral HPLC

Size Exclusion

  • GPC
  • GFC

Ion Chromatography

  • Anions
  • Cations

Ion Exchange Chromatography

Hydrophobic Interaction Chromatography

Affinity Chromatography

Columns for Protein Separations

Columns for sugar analysis

Column Protection

  • Use of precolumns & guard columns
  • Mobile Phase & Sample Filtration
  • In-Line Filters

Column Regeneration

  • Washing off contaminants
  • Changing frits
  • Filling Voids

Five uses for an old column!

Scale up to Prep and semi-prep scale

  • Calculating column size required
  • Calculating new flow rate
  • System changes - pump heads, injection valve, tubing, autosampler, flow cell etc
  • Method development
  • Solvent recycling
  • Peak collection
 
 
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Course Dates in 2010

 
  9th September Manchester  
  12th November Edinburgh  
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