Masterclass in Modern HPLC

Masterclass 2 - HPLC Columns

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	-Masterclass 1 - The Science behind HPLC
	-Masterclass 2 - HPLC Columns
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This module is part of the Masterclass in Modern HPLC programme. This is a rolling programme of six modules, which are run once every two months at six locations around the UK. Each module is complete in isolation, and there is no need to do the modules in any particular order. Simply select your preferred location, and in the following 12 months you will complete the course.
Duration:	1 day
Cost:	£250 + VAT when taken alone. Book the full Masterclass in Modern HPLC and pay just £1095.00 + VAT for all six modules!
Suitable for:	Anyone with at least 6 months experience with HPLC
Locations Available:	Birmingham Bristol Cambridge Edinburgh Manchester London
Course Presenter:	Dr Stuart Jones

Summary:
This module gives an overview of the columns available in HPLC. It is not a guide to column manufacturers although many brands of column are referenced in the course. The aim is to enable an HPLC analyst to be able to make an intelligent choice when selecting columns for a specific application.
Course Content:
Column Packing Material Silica Alumina Polymers Zirconia Carbon Monolithic silica Particle Size Bonded phases C18/ODS C8 C4/C6/C1 End Capping Polar Embedded Phases Amino Cyano DIOL Base Deactivated Phases Normal Phase and reversed Phased Using Polymer-based Reversed Phase columns Column Sizes and Shapes Column Loading Capacity Scale down to microbore & narrowbore Chiral HPLC Size Exclusion GPC GFC Ion Chromatography Anions Cations Ion Exchange Chromatography Hydrophobic Interaction Chromatography Affinity Chromatography Columns for Protein Separations Columns for sugar analysis Column Protection Use of precolumns & guard columns Mobile Phase & Sample Filtration In-Line Filters Column Regeneration Washing off contaminants Changing frits Filling Voids Five uses for an old column! Scale up to Prep and semi-prep scale Calculating column size required Calculating new flow rate System changes - pump heads, injection valve, tubing, autosampler, flow cell etc Method development Solvent recycling Peak collection

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	Course Dates in 2010
	9th September	Manchester
	12th November	Edinburgh
	Course Accreditation for CPD Points
	Royal College of Pathologists
	Institute of Biomedical Scientists
	Institute of Science & Technology
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	Free Lunchtime Seminars