AdvancedHPLC

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This module gives an overview of the columns available in HPLC. It is not a guide to column manufacturers although many brands of column are referenced in the course. The aim is to enable an HPLC analyst to be able to make an intelligent choice when selecting columns for a specific application.

What are eleven reasons why reversed phase has almost totally replaced normal phase chromatography? How does HILIC work? How can we get the best performance from one of the new fused core columns? Where does uHPLC fit in? When might we use the new pentafluorophenyl phase? When should we use C1 or C4 columns? What are the three major benefits of a phenyl column? When might zirconia be really useful as a stationary phase? Why might we consider a monolithic column packing? What should we change when switching from a silica-based column to a polymer-based column? What are the requirements for an eluent for GPC? How can we protect the column from an agressive mobile phase? All these and many more are covered in this course, including five uses for an old column!