Masterclass in Modern HPLC

Masterclass 1 - The Science behind HPLC

sales@hplccourses.com
 
  Laserchrom Home Page  
  HPLC Courses Home Page  
  Practical HPLC Courses at Laserchrom  
  Introduction to Practical HPLC  
  Introduction to Practical GPC  
  Intermediate Practical HPLC  
  Advanced Practical HPLC  
  HPLC Method Development  
  Practical HPLC Troubleshooting  
  HPLC Equipment Servicing  
  AGILENT HPLC Equipment Servicing  
  LC-MS Data Interpretation  
  Clarity Data System  
  HPLC in Hospital Laboratories  
  Amino Acid Analysis  
  HPLC of Sugars  
  Off-site Courses around the UK  
  Introduction to HPLC  
  Introduction to GPC  
  Introduction to Ion Chromatography  
  Masterclass in Modern HPLC  
   -Masterclass 1 - The Science behind HPLC  
   -Masterclass 2 - HPLC Columns  
   -Masterclass 3 - HPLC Detection  
   -Masterclass 4 - Eluent Optimisation  
   -Masterclass 5 - Integration & Calibration  
   -Masterclass 6 - HPLC Instrumentation  
  HPLC Troubleshooting  
  LCMS Data Interpretation  
  On-site Courses in your company  
     
 
 
     
 

This module is part of the Masterclass in Modern HPLC programme.

This is a rolling programme of six modules, which are run once every two months at six locations around the UK. Each module is complete in isolation, and there is no need to do the modules in any particular order. Simply select your preferred location, and in the following 12 months you will complete the course.

Duration: 1 day
Cost:

£250 + VAT when taken alone.

Book the full Masterclass in Modern HPLC and pay just £1095.00 + VAT for all six modules!

Suitable for: Anyone with at least 6 months experience with HPLC

Locations Available:

Birmingham

Bristol

Cambridge

Edinburgh

Manchester

London

Course Presenter: Dr Stuart Jones
 

Summary:

This module gives an overview of the theory of HPLC, and how its application can help an analyst save time every day. It explains how things happen, why peaks elute when they do, and what does and what doesn't improve an HPLC separation!
Course Content:

HPLC Theory

Relative retention time

Selectivity

Efficiency

The Resolution Equation

The van Deemter equation

Peak Asymmetry

Effect of peak size ratio on overlap

Relationship between k' and resolution

Effect of changing k' on selectivity

Effect of changing solvent type

Optimising Efficiency

Thermodynamic properties

  • Retention times
  • Selectivity

Kinetic Properties

  • Five processes contributing to band broadening
  • Efficiency

Importance of particle size

Principles behind UPLC

Solvophobic Theory

Acid - Base Equilibria

Understanding Isocratic Elution

The usefulness of the void volume peak

Factors affecting peak retention time

  • Solvent temperature
  • Column temperature
  • Eluent pH
  • Buffer salt
  • Buffer concentration
  • Organic modifier in the eluent
  • Eluent %B
  • Column type or chemistry
  • The distribution constant

The effect of changing column length

The effect of changing column width

Maintaining linear velocity when changing column diameter

Understanding Gradient Elution

The new Resolution equation for gradient elution

Gradient pre-elution

Gradient mixing volume

Gradient dwell volume

The Knox equation

Extra-column void volume

When to use a gradient

Which columns to use with gradient elution

The importance of gradient slope

The average k' parameter and how to change it.

The importance of the gradient composition range

What happens if we use the wrong gradient range?

  • Initial %B too high
  • Final %B too low
  • Effect of changing flow rate

Optimising column dimensions, flow rate and particle size for resolution, run time or back pressure

 
 
  Customer Comments  
 

Course Dates in 2010

 
  10th September Edinburgh  
  10th November Birmingham  
  Course Accreditation for CPD Points  
  Royal College of Pathologists  
  Institute of Biomedical Scientists  
  Institute for Science & Technology  
  Travel and Accommodation  
  Accommodation information  
  Booking Information  
  Course Calendar  
  Booking Form  
  Terms and Condition of Booking  
  Free Lunchtime Seminars