Masterclass in Modern HPLC
Masterclass 1 - The Science behind HPLC
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This module is part of the Masterclass in Modern HPLC programme. This is a rolling programme of six modules, which are run once every two months at six locations around the UK. Each module is complete in isolation, and there is no need to do the modules in any particular order. Simply select your preferred location, and in the following 12 months you will complete the course. |
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| Duration: | 1 day |
| Cost: | £250 + VAT when taken alone. Book the full Masterclass in Modern HPLC and pay just £1095.00 + VAT for all six modules! |
| Suitable for: | Anyone with at least 6 months experience with HPLC |
Locations Available: |
Birmingham Bristol Cambridge Edinburgh Manchester London |
| Course Presenter: | Dr Stuart Jones |
Summary: |
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| This module gives an overview of the theory of HPLC, and how its application can help an analyst save time every day. It explains how things happen, why peaks elute when they do, and what does and what doesn't improve an HPLC separation! | |
| Course Content: | |
HPLC Theory Relative retention time Selectivity Efficiency The Resolution Equation The van Deemter equation Peak Asymmetry Effect of peak size ratio on overlap Relationship between k' and resolution Effect of changing k' on selectivity Effect of changing solvent type Optimising Efficiency Thermodynamic properties
Kinetic Properties
Importance of particle size Principles behind UPLC Solvophobic Theory Acid - Base Equilibria Understanding Isocratic Elution The usefulness of the void volume peak Factors affecting peak retention time
The effect of changing column length The effect of changing column width Maintaining linear velocity when changing column diameter Understanding Gradient Elution The new Resolution equation for gradient elution Gradient pre-elution Gradient mixing volume Gradient dwell volume The Knox equation Extra-column void volume When to use a gradient Which columns to use with gradient elution The importance of gradient slope The average k' parameter and how to change it. The importance of the gradient composition range What happens if we use the wrong gradient range?
Optimising column dimensions, flow rate and particle size for resolution, run time or back pressure |
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Course Dates in 2010 |
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| 10th September | Edinburgh | ||
| 10th November | Birmingham | ||
| Course Accreditation for CPD Points | |||
| Royal College of Pathologists | |||
| Institute of Biomedical Scientists | |||
| Institute for Science & Technology | |||
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| Accommodation information | |||
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| Course Calendar | |||
| Booking Form | |||
| Terms and Condition of Booking | |||
| Free Lunchtime Seminars | |||